Research into Antibacterial Effects of Polysaccharide-Based Microparticles

Background: Due to worldwide increases in the prevalence of antibiotic-resistant bacteria, it is necessary develop an active drug delivery system that can enable therapeutics reach their molecular targets. Maintaining concentration any blood at a certain level for long time critical practice therapy. With increased frequency use, drugs exceeds therapeutic level, leading toxicity or ineffectiveness. To solve these problems, recent years, much attention has been given developing micro/nano preparations by encapsulating biologically compounds on polymeric carriers. Therefore, we aimed extract pectin from sea buckthorn peel, prepare microcapsules containing antibiotics, and determine physical chemical properties. Methods: Wastes were separated under “Medical raw materials Dry fruit Hippophae rhamnoides MNS 5225:2002”. Pectin was isolated waste according “method determination pectins MNS3080:1981” standard. The degree esterification determined ISO 7623:2016. Antibiotic encapsulation with coacervates water-based emulsions performed. sensitivity microdilution Clinical Laboratory Standard Institute (МТ100-S27) method. results between standard strains Staphylococcus aureus ATCC 29213 MRSA 2758 different dilution concentrations. Result: brown powder sour taste no odor. There 71.4% pectin, 8.9% yield, 1.3% free carboxyl group, 3.2% methylated 4.5% total 3.5% ash, 0.1% nitrogen. A study antibacterial activity doxycycline hyclate found inhibition bacterial growth 0.8 times less than pure pectin. It 1 alone, 33 smaller wontaxime when compared inhibited 6 lower This 2 8 wontaxime. Conclusion: yields 1.3 after 60 minutes separation sediment ratio 1:1.15 pH = 2. itself against MRSA.